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Using bright-field microscopy to look at a slide prepared with a basic dye you observe cells with a clear inner compartment within the cell at 400X magnification. The cell is most likely a(n)


A) prokaryote.
B) bacterium.
C) archaeon.
D) eukaryote.

E) B) and D)
F) A) and B)

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A bacterial cell is interpreted as gram-positive when it forms purple insoluble crystal violet-iodine complexes within the cell during the Gram stain.

A) True
B) False

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Some archaea have unique phospholipids in their cytoplasmic membrane that


A) form a monolayer due to the presence of diglycerol tetraethers.
B) form a bilayer due to the presence of sterols.
C) form a stable ring structure due to the presence of crenarchaeol.
D) form a bilayer due to the presence of phosphatidylethanolamine.

E) None of the above
F) B) and C)

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Membrane-enclosed organelles, such as nuclei, lysosomes, endoplasmic reticulum, mitochondria, and chloroplasts


A) form specialized compartments within eukaryotic cells for specific functions to occur.
B) increase the structural complexity of eukaryotic cells.
C) help large eukaryotic cells overcome the limitations of diffusion imposed by their large cell size.
D) increase structural complexity, help eukaryotes overcome diffusion limitation due to their size, and form specialized environments for specific functions to occur.

E) None of the above
F) A) and D)

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You have discovered a new bacterial strain that causes urinary tract infections. Closely related bacterial species cannot cause infections. You compare the strains and find that your new strain has structures composed of protein external to its cell wall. What structures might your new strain have that the other strains do not? Why?

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Pili or fimbrae are the most likely stru...

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An organism of the genus Staphylococcus is ________, while an organism of the genus Spirochaeta is ________.


A) spherical / rod shaped
B) rod shaped / coiled
C) spherical / coiled
D) coiled / spherical

E) C) and D)
F) None of the above

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Some of the intestinal symptoms elicited by pathogens such as Salmonella, Shigella, and Escherichia are due to the presence of


A) pseudomurein.
B) S-layers.
C) lipopolysaccharides.
D) peptidoglycan.

E) A) and B)
F) A) and C)

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Eukaryotes have ________ in their cytoplasmic membranes, which serve to strengthen and stabilize the membrane and make it less flexible. Many bacteria have similar molecules, known as ________, in their cytoplasmic membranes that have a similar role.


A) ether bonds / ester bonds
B) lipids / phospholipids
C) sterols / hopanoids
D) phospholipids / lipopolysaccharides

E) A) and C)
F) None of the above

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The energy source derived from the charge separation across the cytoplasmic membrane is referred to as


A) the proton motive force.
B) carbohydrate charging.
C) adenosine triphosphate.
D) the voltage source.

E) A) and B)
F) A) and C)

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Predict what would happen to a motile bacterium undergoing chemotaxis if the Mot proteins suddenly ceased to function.

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The function of Mot proteins should be d...

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Cellular inclusions in prokaryotic cells serve to


A) store energy rich compounds.
B) protect DNA.
C) position cells in the appropriate environment for survival.
D) store energy rich compounds and position cells in the appropriate environment for survival.

E) All of the above
F) A) and B)

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Smaller prokaryotic cells generally grow faster than larger ones due to a higher surface-area-to-volume ratio.

A) True
B) False

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The peptide interbridge crosslinking between peptidoglycan layers is found ONLY in the cell walls of


A) Archaea.
B) Eukarya.
C) gram-positive Bacteria.
D) gram-negative Bacteria.

E) A) and C)
F) A) and B)

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What is the function of an endospore and how is an endospore formed?

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The function of an endospore is to allow...

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The use of the Gram stain in microbiology is important because it differentiates


A) Bacteria from Archaea.
B) prokaryotic from eukaryotic cells.
C) bacterial cells with different types of cell walls.
D) archaeal cells with different types of metabolism.

E) A) and D)
F) B) and D)

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The Golgi complex functions to


A) modify and secrete proteins to the external environment.
B) sort proteins used within the cell.
C) both modify and sort proteins into those destined for secretion and those that function in membrane structures.
D) synthesize proteins.

E) B) and C)
F) A) and C)

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Based on the table of attributes given below, which of the following statements are FALSE about the two organisms?  Characteristic  Bacterium A  Bacterium B  Endospore formation  yes  no  Capsule  no  yes  Type IV pili  yes  no  Flagella  no  no  Morphology  bacillus  bacillus \begin{array}{lll}\text { Characteristic } & \text { Bacterium A } & \text { Bacterium B } \\\hline \text { Endospore formation } & \text { yes } & \text { no } \\\text { Capsule } & \text { no } & \text { yes } \\\text { Type IV pili } & \text { yes } & \text { no } \\\text { Flagella } & \text { no } & \text { no } \\\text { Morphology } & \text { bacillus } & \text { bacillus }\end{array}


A) Bacterium A is more resistant to heat and ultraviolet light.
B) Bacterium B likely forms a slime layer better than Bacterium A.
C) Bacterium B is likely to exhibit motility.
D) Both bacteria may attach to surfaces.

E) B) and D)
F) A) and B)

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In general, most cell inclusions function as energy reserves or as a reservoir of structural building blocks.

A) True
B) False

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Bacteria stain as gram-positive or gram-negative because of differences in the cell


A) wall.
B) cytoplasm.
C) nucleus.
D) chromosome.

E) A) and D)
F) None of the above

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The lipids in the cytoplasmic membrane of Bacteria and ________ contain ester linkages, while the cytoplasmic membrane of ________ contain ether linkages.


A) Archaea / Eukarya
B) Archaea / fungi
C) Eukarya / prokaryotes
D) Eukarya / Archaea

E) A) and D)
F) A) and C)

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